This guide outlines how researchers reconstitute lyophilized LL-37 into a solution of known concentration for laboratory use. It is reference information for preparing research solutions only. LL-37 is sold strictly for in-vitro laboratory research and is not for human or veterinary use, consumption, or diagnostic application.
Concentration is simply compound mass divided by diluent volume. For example, 5 mg reconstituted in 2 mL of diluent gives a solution of 2.5 mg/mL. Use more diluent for a lower concentration, less for a higher one. The reconstitution concentration calculator works this out for any mass and volume.
Store lyophilized powder frozen at -20°C. Keep reconstituted solution refrigerated at 2–8°C and use within your protocol window; avoid repeated freeze–thaw of this longer peptide.
Purity is determined by HPLC and identity is confirmed by mass spectrometry. Each batch ships with a batch-specific certificate of analysis from an independent third-party laboratory; refer to the COA for lot-specific measured values. Endotoxin and sterility testing are not performed unless explicitly stated.
Add bacteriostatic or sterile water slowly down the inner wall of the vial, then swirl gently until clear. Never shake. Example: 5 mg in 2 mL gives a 2.5 mg/mL solution.
The volume only sets the concentration (mass ÷ volume) — more diluent means a lower concentration. Use the reconstitution concentration calculator to find the volume for a target mg/mL.
Keep reconstituted solution refrigerated and use it within the window appropriate to your research protocol. Store unopened lyophilized powder frozen. See the storage section above for specifics.
No. It is sold strictly for in-vitro laboratory research and is not for human or veterinary use.