Semax (Heptapeptide) Reconstitution Guide

This guide outlines how researchers reconstitute lyophilized Semax for laboratory use. It is reference information for preparing research solutions only. Semax is sold strictly for in-vitro laboratory research and is not for human or veterinary use, consumption, or diagnostic application.

What you need

• The lyophilized Semax vial
• Bacteriostatic water or sterile water as diluent
• Alcohol swabs and a sterile syringe

Step by step

• Wipe the rubber septum of both the Semax vial and the diluent vial with a fresh alcohol swab and let them air-dry.
• Draw your chosen volume of diluent into the syringe.
• Inject the diluent slowly, letting it run down the inner glass wall of the vial rather than directly onto the powder.
• Swirl gently to dissolve. Never shake — agitation can shear peptide chains. Let the vial sit until the solution is clear.

Example concentration

A common laboratory preparation is 10 mg reconstituted in 2 mL of diluent, which gives a concentration of 5 mg/mL. Concentration is simply compound mass divided by diluent volume — use more water for a lower concentration, less for a higher one. To compute the draw volume for any target amount, use the reconstitution calculator.

Storage and stability

Store lyophilized powder frozen at -20°C. After reconstitution, keep refrigerated at 2–8°C and use within the window appropriate to your protocol. Avoid repeated freeze–thaw of the reconstituted solution.

Analytical and COA

Purity is determined by HPLC and identity is confirmed by mass spectrometry. Each batch ships with a batch-specific certificate of analysis from an independent third-party laboratory; refer to the COA for lot-specific measured values. Endotoxin and sterility testing are not performed unless explicitly stated.